Western blot analysis of PTCRA in extracts of various cell lines. Samples were incubated with PTCRA Polyclonal antibody (Product # PA5-110236) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Western blot analysis of PTCRA in K-562, Jurkat, HL-60, B cells, THP-1, Raji. Samples were incubated in polyclonal PTCRA antibody (Product # PA5-110236) using a dilution of 1:1000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10000.
Supplier Page from Thermo Fisher Scientific for PTCRA Antibody